cholesterol oxidase การใช้

• As a excellent material for preparation of biomembrane , cellulose acetate ( ca ) is a fine carrier for the immobilization of cholesterol oxidase ( cod )
• Therefore , research on cholesterol oxidase has become more and more important . a 1 . 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi . 4 - 2g2
• Because of its high toxity activity and complementary inseticidal spectrum with bt . endotoxin which is now being used universally , the cholesterol oxidase is acknowleged as one of the representitive of the insecticidal protein of the second generation
• As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
• Moreover , together with cholesterol esterase and catalyse , cholesterol oxidase can be used as test reagent to determine the total cholesterol content in blood plasma for the diagnosis of such as cardiovascular reflex and atherosclerosis clinically
• The expression mixture were eletrophoresized by sds - page , revealing a specific 56 kd protein in bl21 expression system , which is of the same size in expectation . a colorimetric assay for measuring cholesterol oxidase showed the cholesterol oxidase activity of the expression product
• Cholesterol oxidase is a type of protein that was found to have pest - resistant activity only years ago , its toxity to such sensitive pests as cotton boll weevil is euqivalent to previously found bt . endotoxins . the protein can also inhibit the growth and development of cotton boll worm
• In this study , a new gene c / wew , encoding cholesterol oxidase , was isolated from rhodococcus equi . 4 - 2g2 found in china , which may be useful in clinical diagnosis healthy food and pest management in agriculture . in addition , the gene has been expressed successfully , the expression product has cholesterol oxidase activity , thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
• The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4 . 0 and 11 . 5 . the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state . when streptomyces griseus atcc14811 was cultured in 10 . 3 % sucrose yeme liquid medium , production of extracellular cholesterol oxidase increased for 5 days before decrease
• The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size . computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70 . 3 % that would encode a protein of 552 amino acids ( aa ) . the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
• The precursor of cholesterol oxidase of atcc14811 was predicted with a calculated mr of 60020 . the cholesterol oxidase produced by streptomyces griseus atcc14811 was purified from the culture broth by procedures including steps for preparation of crude enzyme solution , ammonium sulfate fraction and column chromatography on deae - cellulose
• Air of this advanced development provides efficient methods for protein engineering . in this study , a new two - step procedure has been developed for the detection of cholesterol oxidase activity in streptomyces strains . streptomyces griseus atcc14811 was confirmed to produce cholesterol oxidase and it also showed homologies with the cholesterol oxidase gene ( choa ) from streptomyces sp